The Activation of GLUT1, AMPKα and PPARγ by in L6 Myotubes GLUT1, AMPKα ve PPARγ’nın L6 miyotübülerinde tarafından aktivasyonu
نویسندگان
چکیده
AIM: ( , TC) has long been used in traditional medicine in South East Asia for the treatment of type 2 diabetes mellitus. In the present study, it was aim to investigate the effect of water-ethanol extract of (WETC) on glucose transporter (GLUT1 and GLUT4), 5'AMP-activated protein kinase (AMPKα1) and peroxisome proliferator-activated receptors (PPARγ) expression. METHODS: The activation of GLUT1, GLUT4, AMPKα1 and PPARγ by were assessed by the treatment of WETC to L6 myotubes followed by determined these genes expression using reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: WETC at 400 μg/ml significantly increased glucose uptake at 24 hours (214.49 ± 9.00%, <0.05). In addition, WETC significantly increased GLUT1, AMPKα1 and PPARγ mRNA levels ( <0.05). CONCLUSION: In summary, our results suggest that the enhancement of glucose transport by in L6 myotubes is mediated by the up-regulation of GLUT1, AMPKα and PPARγ expression.
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